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Normal Human Primary Renal Proximal Tubular Epithelial Cells (Rptec, Lonza Catalog #Cc 2553), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
Human Renal Proximal Tubular Epithelial Cells Rptecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal renal proximal tubular epithelial cell (rptec)  (Lonza)
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Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
Normal Renal Proximal Tubular Epithelial Cell (Rptec), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+renal+proximal+tubular+epithelial+cells+(rptec)/human+renal+proximal+tubule+epithelial+cells/pm34965300-69-0-10
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normal renal proximal tubular epithelial cell (rptec) - by Bioz Stars, 2026-08
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Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
Renal Proximal Tubular Epithelial Cells Rptec, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+renal+proximal+tubular+epithelial+cells+(rptec)/Primary+Renal+Proximal+Tubule+Epithelial+Cells%3B+Normal%2C+Human/pm31918226-65-0-7
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Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
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renal proximal tubular epithelial cells rptecs  (ATCC)
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Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
Renal Proximal Tubular Epithelial Cells Rptecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+renal+proximal+tubular+epithelial+cells+(rptec)/Primary+Renal+Proximal+Tubule+Epithelial+Cells%3B+Normal%2C+Human/pm29362864-51-0-10
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renal proximal tubular epithelial cells rptecs - by Bioz Stars, 2026-08
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normal human renal proximal tubular epithelial cells (rptecs)  (Lonza)
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Lonza normal human renal proximal tubular epithelial cells (rptecs)
Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
Normal Human Renal Proximal Tubular Epithelial Cells (Rptecs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+renal+proximal+tubular+epithelial+cells+(rptec)/human+proximal+tubule+cells/pmc03448772-73-2-20
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Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of RPTECs and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular epithelial cell. *p < 0.05, ***p < 0.001.

Journal: Kidney research and clinical practice

Article Title: Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.

doi: 10.23876/j.krcp.21.303

Figure Lengend Snippet: Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of RPTECs and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular epithelial cell. *p < 0.05, ***p < 0.001.

Article Snippet: Human renal proximal tubular epithelial cells (RPTECs) were cultured in American Type Culture Collection (ATCC)-formulated DMEM/F12 supplemented with an hTERT RPTEC Growth Kit (ATCC, Manassas, VA, USA) as previously described [21].

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Western Blot, Enzyme-linked Immunosorbent Assay